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Vector borne Disease

Vector borne Disease

Vector borne Disease

106 - An Anopheles Affliction: The Challenges of Collecting, Rearing, and Colonizing Field Strains of Anopheles quadrimaculatus and Anopheles crucians in a Laboratory Setting

Wednesday, March 25, 2026
8:50 AM - 9:00 AM PDT
Location: B119
Abstract: With the emergence of several locally acquired cases of malaria in Florida in 2023, increased Anopheles mosquito surveillance became the forefront of operational importance in Manatee County, FL. Analyses of pre- and post- mission trapping efforts revealed abnormalities in effective field control of adult Anopheles quadrimaculatus and Anopheles crucians. From this finding, the necessity of resistance testing local populations of Anopheles spp. in the laboratory became clear. Through trial, error, and two years of studying the behaviors of Anophelines in both adult and larval form, we successfully obtained bottle bioassay resistance testing data for deltamethrin and malathion as well as bred and maintained a colony of An. quadrimaculatus to the F6 generation.