Director of Research and Surveillance Pasco County Mosquito Control District, Florida, United States
Abstract: Standard bioassay methodologies often face challenges from the equipment used, including potential interactions between test substances and container materials. Such interactions can introduce procedural artifacts, leading to inaccurate data that can compromise surveillance and operational decisions.
To address this, we evaluated an alternative bioassay containment system fabricated from a material with properties designed to minimize test interference. We conducted parallel laboratory assays comparing this new system against standard methods using common chemical control agents.
Furthermore, the system was adapted for operational field assessments to determine its suitability for in-situ efficacy trials. The alternative system demonstrated improved data fidelity, reduced variability, and offered significant operational advantages in terms of durability and long-term use. This system offers a robust alternative to conventional methods, improving the reliability of entomological assessments.
