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Insecticide Resistance I

Insecticide Resistance I

Insecticide Resistance I

285 - Calibrating the CDC Bottle Bioassay for Malathion and Bifenthrin Using MSU Ae. triseriatus

Thursday, March 26, 2026
2:35 PM - 2:45 PM PDT
Location: B115
Abstract: La Crosse virus (LACV) is an encephalitic arbovirus that primarily affects children under the age of 16 in the southeastern region of the United States. LACV's primary vector is Aedes triseriatus, more commonly known as the eastern treehole mosquito. Ae. triseriatus has a wide distribution, being found in most of the eastern half of the United States. Though this mosquito species has epidemiological importance, the insecticide resistance of this species remains largely uncharacterized. This study aims to establish diagnostic doses and times for both malathion and bifenthrin for Ae. triseriatus using the Centers for Disease Control and Prevention (CDC) bottle bioassay. Using laboratory reared 3-5 day old males and females, susceptibility was first evaluated by exposing the mosquitoes to the CDC-recommended diagnostic doses of other Aedes species. Since complete mortality was reached before the diagnostic times of other Aedes species, insecticide concentration was then gradually reduced to find the correct diagnostic dose. The diagnostic doses for Ae. triseriatus were determined to be 10 µg/bottle for malathion and 75 µg/bottle for bifenthrin. Future work will evaluate field populations and additional active ingredients.